Gram-RodCat+Ox-

Oxidase Negative Catalase Positive Gram Negative Rods
The genera present in this category are Acinetobacter, Citrobacter, Enterobacter, Escherichia, Klebsiella, Morganelle, Proteus, Providencia, Salmonella, and Seratia. These will be further divided into several sections based on the results of primarily the TSI media, and the Citrate test. The Nitrate test will also be required to separate Acinetobacter quickly from the rest of the organisms. These tests do not meaningfully separate them by genus, as some genera are split apart by them, but serve to break the large amount of organisms into smaller sections for ease of testing as all of these tests only require 24 hours to show results. It is important to check TSI in 24 hours or less, as it can turn K/A over time regardless of organism.

Tests
Essential: TSI Agar, Citrate, Nitrate

Key
1-A: The organism does not reduce Nitrate Acinetobacter

1-B: The organism reduces Nitrate to Nitrite (red coloration when reagents are added, but not zinc) GO TO 2

2-A: The organism is K/A (red slant, yellow butt) on TSI GO TO 3

2-B: The organism is A/A (all yellow) on TSI GO TO 4

3-A: [K/A]The organism is Citrate negative GO HERE

3-B: [K/A]The organism is Citrate positive GO HERE

4-A: [A/A]The organism is Citrate negative GO HERE

4-B: [A/A]The organism is Citrate positive GO HERE