TSB

Tryptic Soy Broth is a general purpose broth made of Tryptone, salt, glucose, and several other ingredients. It is also found mixed with agarose to form TSA plates. The media is used as a basis for other identification tests. One such test is a heat tolerance test, it uses typical TSB but incubates it in a 45°C water bath. Most organisms do not grow at this temperature, but cloudy media indicates heat tolerance and growth. Salt can also be added to the media to test salt tolerance. 6.5% salt TSB is used in this guide. Cloudy media indicates growth and salt tolerance positive.