Gram+CocciCat-

Catalase Negative Gram Positive Cocci
These qualities are possessed by the Streptococcus and Enterococcus genera. The list of organisms is S. pyogenes, S. agalactiae, S. intermedius, S. mitis, and E. faecalis

Tests
Required: Blood agar, Bile esculine test, MR-VP, TSB 45°C, TSB 6.5% NaCl, CTA lactose, CTA mannitol

Helpful: Bacitracin resistance test

Key
1-A: Organism is Bile Esculin negative GO TO 2

1-B: Organism is Bile Esculin positive GO TO 3

2-A: Organism is beta hemolytic, MR negative and suceptible to bacitracin S. pyogenes

2-B: Organism is gamma hemolytic, MR positive and not suceptible to bacitracin S. agalactiae

3-A: Organism is mannitol negative, Lactose positive, TSB 45°C positive and TSB salt positive S. intermedius

3-B: Organism is mannitol positive GO TO 4

4-A: Organism is CTA lactose positive, may grow at 45°C S. mitis

4-B: Organism is CTA lactose negative, variable growth in salt and at 45°C E. faecalis