Thiogel

Thiogel is one formulation of the gelatinase experiment. All forms have a large quantity of gelatin, and protein source of some kind (peptone, beef extract, etc.) forming the media into a semi-solid state. Both the needle and loop inoculation methods are fine so long as the organism makes it the length of the tube. This is one of the slower tests, and takes several days of incubation to take full effect. Once removed from incubation, the media will be completely liquified and needs to be chilled again to reform. If the media doesn't fully reform, then the organism has broken down the gelatin and is a positive organism, no change after four days is considered negative.