OF Media

This media is produced with a large quantity of a single sugar, peptone, bromthymol blue(green at high pH, yellow at low), and sometimes a low concentration of agarose. The large sugar:peptone ration keeps peptone from having a large influence on media pH. This media is made with a large number of different sugars, though glucose, sucrose, and lactose are very common choices. This test was designed to see the difference in sugar utilization between oxic and anoxic states, and because of this, it is performed in pairs. One tube will be open, and the other closed off with sterile mineral oil. Fermentation can occur in either tube, but oxidative sugar processing can only work in the open tube. Both tubes positive will mean fermentation is definite, and oxidation is possible. Closed positive open negative indicates obligate anaerobe. Closed negative, open positive, means obligate aerobe. Both inert means the organism cannot utilize the sugar in a way testable by this media.